https://hochschildlab.med.harvard.edu/landing-bedford daily 1.0 2025-08-08 https://images.squarespace-cdn.com/content/v1/60915ef161e00000bf58917d/1620141178311-23AD31WP9ZTPDQKH99Q6/AHochschildLG.jpg Home Meet the team doing the work. https://images.squarespace-cdn.com/content/v1/60915ef161e00000bf58917d/1620332966006-4SIFH5V5N32VGOPCXHT2/Picture3.png Home Find out more about what we do in the lab. https://images.squarespace-cdn.com/content/v1/60915ef161e00000bf58917d/1620140714580-RBYJHNR3663Z5IHPG50U/cover4.jpg Home https://hochschildlab.med.harvard.edu/research daily 0.75 2025-08-08 https://images.squarespace-cdn.com/content/v1/60915ef161e00000bf58917d/1620397476731-MLYYBW000FST0OC8SZL2/Picture1.jpg Research Fluorescence and phase contrast images of dividing E. coli cells containing a laboratory constructed rod-forming GFP fusion protein (see McPartland et al., Nat Struct Mol Biol, 2018). https://images.squarespace-cdn.com/content/v1/60915ef161e00000bf58917d/1620150168727-NZ8XCHWOJ9V2UUJC5I9L/cover4.jpeg Research E. coli cells programmed to produce infectious, extracellular amyloid fibrils of a yeast prion protein (see Sivanathan & Hochschild, Genes Dev, 2012). https://images.squarespace-cdn.com/content/v1/60915ef161e00000bf58917d/1620150174797-TBGOP8088YGOEOQ4QT48/Picture3.png Research A global regulator from the bacterium Clostridium botulinum (Cb-Rho) exhibits hallmark characteristics of prion-forming proteins, assembling as amyloid aggregates when secreted by E. coli cells (left) and adopting alternative conformations when produced in the E. coli cytoplasm, with pale and dark blue colonies signaling the nonprion and prion conformations, respectively (right) (Yuan & Hochschild, Science, 2017). https://images.squarespace-cdn.com/content/v1/60915ef161e00000bf58917d/1620150183952-93FUID2PKRR8P6IWS4EB/Picture1+copy.png Research Transcription-based bacterial two-hybrid assay detects protein-protein interaction between protein domain X fused to RNA polymerase (RNAP) and partner domain Y fused to a DNA-binding protein. Use of assay to genetically dissect interaction between RNAP β-flap domain and region 4 of σ subunit. Structure shows location of isolated substitutions that alter interaction strength (Nickels et al., PNAS, 2005). https://images.squarespace-cdn.com/content/v1/60915ef161e00000bf58917d/1620150241608-7PW1CJWT7R34CZHVAESU/Goldman_Fig5_main_FINAL%5B1%5D.png Research Cartoon depicts how the σ subunit of RNAP can induce transcription pausing by engaging promoter-like motifs within transcription units. σ-dependent pausing occurs via two pathways: either the initiating σ factor is retained (top) or σ accesses the transcription elongation complex in trans (bottom), enabling two different σ factors to control initiation and elongation on the same transcription unit (Goldman et al., eLife, 2015). https://images.squarespace-cdn.com/content/v1/60915ef161e00000bf58917d/1620150294638-FDBAVYEUCATCOGPHGOYN/Screen+Shot+2018-08-22+at+2.21.41+PM.png Research Fluorescence image showing E. coli cells containing twisted ribbon structures formed by a transplanted yeast prion-forming protein fused to YFP (Garrity et al., PNAS, 2010). https://hochschildlab.med.harvard.edu/people daily 0.75 2025-07-07 https://images.squarespace-cdn.com/content/v1/60915ef161e00000bf58917d/1620403475210-IN1HG8A3X40SDI5V8XTC/EmilyKate+McDonough.jpg People - Make it stand out EMILYKATE MCDONOUGH, Project Manager, Tufts University School of Medicine https://images.squarespace-cdn.com/content/v1/60915ef161e00000bf58917d/1620158334596-97D8TEY3NAURRRYHGGK3/AHochschild2018.jpg People - ANN HOCHSCHILD, PI https://images.squarespace-cdn.com/content/v1/60915ef161e00000bf58917d/1620157737980-K16CDRZ3QB90VRVOS45R/Padraig.jpg People - PADRAIG DEIGHAN https://images.squarespace-cdn.com/content/v1/60915ef161e00000bf58917d/1620154938410-HARAJ8REHDZ1R0V00TNE/ZoeFeder.jpeg People - ZOË FEDER https://images.squarespace-cdn.com/content/v1/60915ef161e00000bf58917d/1620157777195-G8RT8G7N0WEH5IE0EMQZ/Ellie.jpg People - ELEANOR FLEMING https://images.squarespace-cdn.com/content/v1/60915ef161e00000bf58917d/1620158351160-KKIPONWO0KJHU9PCUZNF/Kemardo%25252Bcopy.jpg People - KEMARDO HENRY https://images.squarespace-cdn.com/content/v1/60915ef161e00000bf58917d/1751912556335-R4IOMJBRUN6E3N31D4YO/F7ECAC5A-5D3B-491F-A993-3EC0ECE49914.JPG People - KRISTOPHER KENNEDY https://images.squarespace-cdn.com/content/v1/60915ef161e00000bf58917d/1620158568290-9XN5OUFG2L0E8GKP894E/Zenaty2.jpg People - DAVID ZENATY https://images.squarespace-cdn.com/content/v1/60915ef161e00000bf58917d/1620399512345-15FSRIMU1IAMKLYQDLZ0/SethGoldman.jpeg People SETH RAPHAEL GOLDMAN, Core Director, Nascent Transcriptomics Core, HMS https://images.squarespace-cdn.com/content/v1/60915ef161e00000bf58917d/1620399620980-P6XQHDSMYWLAMMWDGR9G/AndyYuan.png People ANDY YUAN, Postdoctoral Fellow at Harvard Medical School https://images.squarespace-cdn.com/content/v1/60915ef161e00000bf58917d/1620399654403-LBZQ01BX51KV85LBLWE2/Heller.png People DANIELLE HELLER, Science Education Fellow at HHMI https://images.squarespace-cdn.com/content/v1/60915ef161e00000bf58917d/1620403738033-LIX9FR75EX2EOSV1WAF3/Hanif.jpg People - Make it stand out HANIF VAHEDIAN MOVAHED https://images.squarespace-cdn.com/content/v1/60915ef161e00000bf58917d/1620404097480-E3NFQPI5W9ZV51KZUF72/Springstein.jpg People - Make it stand out BENJAMIN SPRINGSTEIN, IST-BRIDGE Postdoctoral Fellow, Institute of Science and Technology, Austria https://hochschildlab.med.harvard.edu/publications daily 0.75 2025-07-14 https://images.squarespace-cdn.com/content/v1/60915ef161e00000bf58917d/1620313573529-Z9K77O9Q8M3I31JWVKX3/Dec_1_GAD.png Publications https://hochschildlab.med.harvard.edu/ann-hochschild daily 0.75 2025-03-21 https://images.squarespace-cdn.com/content/v1/60915ef161e00000bf58917d/1620400965592-Y3T88S9VZPFS8MV09VBZ/Ann.png Ann Hochschild https://images.squarespace-cdn.com/content/v1/60915ef161e00000bf58917d/1620400648308-ZUCQJ4CC1JGE0CZYXXXW/Hochschild_Headshot.JPG Ann Hochschild https://hochschildlab.med.harvard.edu/padraig-deighan daily 0.75 2021-06-10 https://images.squarespace-cdn.com/content/v1/60915ef161e00000bf58917d/1620401503946-3LSP98YCX8EGL5H3L9IH/Padraig+.png Padraig Deighan https://images.squarespace-cdn.com/content/v1/60915ef161e00000bf58917d/1620401453501-EEJ769DUNHTDQNB57E6C/Padraig.jpg Padraig Deighan https://hochschildlab.med.harvard.edu/zoe-feder daily 0.75 2021-06-10 https://images.squarespace-cdn.com/content/v1/60915ef161e00000bf58917d/1620402923030-OYS8NC2BNCUPN9KCTA7W/ZoeFeder.jpeg Zoë Feder https://images.squarespace-cdn.com/content/v1/60915ef161e00000bf58917d/1620402969315-5ND9C1FC8WWHIQRTGTEV/Zoe+email+image.png Zoë Feder https://hochschildlab.med.harvard.edu/eleanor-fleming daily 0.75 2021-05-07 https://images.squarespace-cdn.com/content/v1/60915ef161e00000bf58917d/1620403198011-W27SA5O6IL53V9EI34ZK/Ellie.png Eleanor Fleming https://images.squarespace-cdn.com/content/v1/60915ef161e00000bf58917d/1620403140973-A4J021OL0B9MLZ8VX3VA/Ellie.JPG Eleanor Fleming https://hochschildlab.med.harvard.edu/kemardo-henry daily 0.75 2022-10-19 https://images.squarespace-cdn.com/content/v1/60915ef161e00000bf58917d/1620403295579-13UNQJBFJ08BYR07ARI6/Kemardo+copy.jpg Kemardo Henry https://images.squarespace-cdn.com/content/v1/60915ef161e00000bf58917d/1620403371306-DR3CJ1U3H3592RJGTB3T/Kemardo+email+image.png Kemardo Henry https://hochschildlab.med.harvard.edu/emilykate-mcdonough daily 0.75 2021-05-07 https://images.squarespace-cdn.com/content/v1/60915ef161e00000bf58917d/1620403475210-IN1HG8A3X40SDI5V8XTC/EmilyKate+McDonough.jpg Emilykate McDonough https://images.squarespace-cdn.com/content/v1/60915ef161e00000bf58917d/1620404292599-UED3JGJ2N8M9QGDHDLDP/Screen+Shot+2019-09-27+at+12.29.06+PM.png Emilykate McDonough https://hochschildlab.med.harvard.edu/hanif-vahedian-movahed daily 0.75 2024-07-24 https://images.squarespace-cdn.com/content/v1/60915ef161e00000bf58917d/1620403828170-HEI0NZQT0YBGLGZ2N47B/Hanif+email+image.png Hanif Vahedian Movahed https://images.squarespace-cdn.com/content/v1/60915ef161e00000bf58917d/1620403738033-LIX9FR75EX2EOSV1WAF3/Hanif.jpg Hanif Vahedian Movahed https://hochschildlab.med.harvard.edu/benjamin-springstein daily 0.75 2021-05-07 https://images.squarespace-cdn.com/content/v1/60915ef161e00000bf58917d/1620404512880-AL4IUITK2EMBVBAWWYM9/Benjamin.png Benjamin Springstein https://images.squarespace-cdn.com/content/v1/60915ef161e00000bf58917d/1620404097480-E3NFQPI5W9ZV51KZUF72/Springstein.jpg Benjamin Springstein https://hochschildlab.med.harvard.edu/david-zenaty daily 0.75 2021-06-10 https://images.squarespace-cdn.com/content/v1/60915ef161e00000bf58917d/1620404689079-BG5IES8CD0IZYKGDNT0H/Zenaty2.jpg David Zenaty https://images.squarespace-cdn.com/content/v1/60915ef161e00000bf58917d/1620404768097-KZ7WVMRPUV5YEZET12BY/David.png David Zenaty https://hochschildlab.med.harvard.edu/contact-1 daily 0.75 2021-05-07